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Authors
Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 52-55
Abstract
β-glucosidase (EC 3.2.1.21) was extracted from ischolar_mains of Hordeum vulgare and was purified using ammonium sulphate fractional precipitation and sephadex G-25 chromatography. The molecular weight of enzyme was found in the range of 17-54KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60ºC. Bioethanol was produced from ischolar_mains of Hordeum vulgare.
Keywords
Hordeum vulgare, β-Glucosidase, Purification, Characterization.
